Artificially made for transplantation and treatment of damaged tissues from diseases and injuries
Must be able to attach and deliver cells
Must induce cell proliferation and accelerate growth
Must stimulate cellular response
Must be quick and efficient with wound healing
Must be biocompatible and biodegradable, etc
It is expected to reach USD 328.37 billion in 2027 from USD 109.39 billion in 2019
(CAGR 15.89%)
The global scaffold technology market is valued at USD 728 million in 2018 and is expected to grow rapidly at a CAGR of 10.38%, reaching USD 1,485 million in 2026.
By utilizing spider silk proteins possessing exceptional biological and physical characteristics, it is possible to address the shortcomings of existing metal and bio-scaffolds.
Furthermore, due to its porous structure, it excels in cell attachment and enables effective drug delivery, leading to enhanced cell growth and anticipated effects
in inducing stem cell differentiation. This can be applied to utilize it as a cell graft or bone graft.
Additionally, depending on the transplantation site, it can be freely shaped into various sizes and shapes.
Easy cell attachment
Suitable for cell transplantation and induction of differentiation
Excellent growth of attached cells
Excellent swelling ratio
Convenient for moisture retention, cell penetration, and drug delivery
Suitable material for inducing bone differentiation*
Swelling ratio, compressive strength, porosity and pore size all show the possibility of spider silk protein to be used as scaffolds
(Adequate for cell attach & osteogenic differentiation*)
After constant seeding of cells in the fabricated scaffold, live/dead assay was performed. (live cells show green fluorescence and dead cells show red fluorescence)
It was confirmed that the survival rate was more than 98% in scaffolds containing spider silk protein.`
After 24h of seeding, as the scaffold swelled, it was checked whether the cells were properly attached and permeated evenly.
Cells are more and more evenly attached to PEGDA-cryogel containing spider silk protein. (Confocal imaging)
Alizarin Red S staining showed higher calcium deposition in PEG+spider slik protein cryogel group when compared to control group
Increase of osteogenic gene markers found in experimental group with spider silk protein
Transplantation of Spider silk incorporated scaffold into skull
Growth factor (BMP, Bone Morphogenetic Proteins) loading for bone regeneration
Confirm bone regeneration after 12 weeks (Micro CT, Histology, Immunohistochemistry etc.)
L Negative control R Negative control |
L PEGDA+Slik R PEGDA |
L PEGDA+Slik+BMP R PEGDA+BMP |
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Red keratin and muscle fibers
Blue or green collagen and bone
Light red or pink cytoplasm
Dark brown to black cell nuclei
Hematoxylin stains cell nuclei a purplish blue
Eosin stains the extracellular matrix and cytoplasm pink
A significant enhancement in biodegradability was observed in the group containing 0.1% silk.
MRI imaging revealed a substantial reduction in volume of the implanted scaffold.
Tissue analysis including H&E staining confirmed biocompatibility of all test samples.